Dr Renwick Dobson- Publications
Renwick's UC Research Profile.
Research publications and dissemination
Peer-reviewed journal articles
2011
50) Dobson RCJ, Girón I, Hudson AO. (2011) L,L-diaminopimelate aminotransferase from Chlamydomonas reinhardtii: a target for algaecide development. PLoS One 6(5):e20439.
49) Pearce FG, Dobson RC, Jameson GB, Perugini MA, Gerrard JA. (2011) Characterization of monomeric dihydrodipicolinate synthase variant reveals the importance of substrate binding in optimizing oligomerization. Biochim Biophys Acta. (Accepted Jul 22)
48) Cross PJ, Dobson RCJ, Patchett ML, Parker EJ. (2011) Tyrosine latching of a regulatory gate affords allosteric control of aromatic amino acid biosynthesis. J Biol Chem. 286(12):10216-24.
47) Evans G, Schuldt L, Griffin MD, Devenish SR, Grant Pearce F, Perugini MA, Dobson RC, Jameson GB, Weiss MS, Gerrard JA. (2011) A tetrameric structure is not essential for activity in dihydrodipicolinate synthase (DHDPS) from Mycobacterium tuberculosis. Arch Biochem Biophys. 512(2):154-9
46) Hudson AO, Klartag A, Gilvarg C, Dobson RCJ, Marques FG, Leustek T. (2011) Dual diaminopimelate biosynthesis pathways in Bacteroides fragilis and Clostridium thermocellum. Biochim Biophys Acta. 1814(9):1162-8.
45) Gunn NJ, Gorman MA, Dobson RCJ, Parker MW, Mulhern TD. (2011) Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase. Acta Cryst F 67(Pt 3):336-9.
44) Veldhuis NA, Kuiper MJ, Dobson RCJ, Pearson RB, Camakaris J. (2011) In silico modeling of the Menkes copper-translocating P-type ATPase 3rd metal binding domain predicts that phosphorylation regulates copper-binding. Biometals 24(3):477-87
43) Hudson AO, Girón I, Dobson RCJ (2011) Crystallization and preliminary X-ray diffraction analysis of L,L-diaminopimelate aminotransferase (DapL) from Chlamydomonas reinhardtii. Acta Cryst F 67(Pt 1):140-3
2010
42) Voss JV, Scally SW, Taylor NL, Griffin MDW, Newman JM, Hutton CA, Parker MW, Gerrard JA, Alderton MR, Dogovski C, Dobson RCJ & Perugini MA (2010) Substrate-mediated stabilization of the active tetrameric form of an antibiotic target from Bacillus anthracis. J. Biol. Chem. 285(8):5188-95.
41) Zhu T, Bailey MF, Angley LM, Cooper TF & Dobson RCJ (2010) The quaternary structure of pyruvate kinase type 1 from Escherichia coli at low nanomolar concentrations. Biochimie 92(1):116-20
40) Wubben JM, Dogovski C, Dobson RCJ, Codd R, Gerrard JA, Parker MW, Perugini MA. (2010) Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the psychrophile Shewanella benthica. Acta Cryst. F 66(Pt 11):1511-6.
39) Muscroft-Taylor AC, Catchpole RJ, Dobson RCJ, Pearce FG, Perugini MA, Gerrard JA. (2010) Disruption of quaternary structure in Escherichia coli dihydrodipicolinate synthase (DHDPS) generates a functional monomer that is no longer inhibited by lysine. Arch Biochem Biophys. 503(2):202-6.
38) Chan KC, Lio DS, Dobson RCJ, Jarasrassamee B, Hossain MI, Roslee AK, Ia KK, Perugini MA, Cheng HC. (2010) Development of the procedures for high-yield expression and rapid purification of active recombinant Csk-homologous kinase (CHK): comparison of the catalytic activities of CHK and CSK. Protein Expr Purif. 74(2):139-47.
37) Soares da Costa TP, Muscroft-Taylor AC, Dobson RCJ, Devenish SR, Jameson GB, Gerrard JA. (2010) How essential is the 'essential' active-site lysine in dihydrodipicolinate synthase? Biochimie 92(7):837-45.
36) Dommaraju S, Gorman MA, Dogovski C, Pearce FG, Gerrard JA, Dobson RCJ, Parker MW & Perugini MA (2010) Cloning, expression and crystallization of dihydrodipicolinate reductase from methicillin-resistant Staphylococcus aureus. Acta Cryst. F 66(Pt 1):57-60.
35) Hor L†, Dobson RCJ†, Dogovski C, Hutton CA & Perugini MA(2010) Crystallization and preliminary X-ray diffraction analysis of diaminopimelate epimerase from Escherichia coli. Acta Cryst. F 66(Pt 1):37-40. † - Joint first author
34) Griffin MD, Dobson RCJ, Gerrard JA & Perugini MA (2010) Exploring the dihydrodipicolinate synthase tetramer: How resilient is the dimer-dimer interface? Arch. Biochem. Biophys. 494(1):58-63.
2009
33) Dobson RCJ, Perugini MA, Jameson GB & Gerrard JA. (2009) Specificity versus catalytic potency: The role of threonine 44 in Escherichia coli dihydrodipicolinate synthase mediated catalysis. Biochimie. 91(8):1036-44.
32) Domigan LJ, Scally SW, Fogg MJ, Hutton CA, Perugini MA, Dobson RC, Muscroft-Taylor AC, Gerrard JA & Devenish SR (2009) Characterisation of dihydrodipicolinate synthase (DHDPS) from Bacillus anthracis. Biochim. Biophys. Acta. 1794:1510-6.
31) Guo BBB, Devenish SRA, Dobson RCJ, Muscroft-Taylor AC & Gerrard JA (2009) The C-terminal domain of Escherichia coli dihydrodipicolinate synthase (DHDPS) is essential for maintenance of quaternary structure and efficient catalysis. Biochem. Biophys. Res. Comm. 380(4):802-6.
30) Voss JV, Scally SW, Taylor N, Dogovski C, Alderton MR, Hutton CA, Gerrard JA, Parker MW, Dobson RCJ & Perugini MA (2009) Expression, purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Bacillus anthracis in the presence of pyruvate Acta Cryst. F 65(Pt 2):188-91. Corresponding author
29) Atkinson SC, Dobson RCJ, Newman JM, Gorman MA, Dogovski C, Parker MW & Perugini MA(2009)Crystallization and preliminary X-ray analysis of dihydrodipicolinate synthase from Clostridium botulinum in the presence of its substrate pyruvate. Acta Cryst. F 65(Pt 3):253-5. Corresponding author
28) James PF†, Dogovski C†, Dobson RCJ†, Bailey MF, Goldie KN, Karas JA, Scanlon DB, O'Hair RA & Perugini MA (2009) Aromatic residues in the C-terminal helix of human apolipoprotein C-I mediate phospholipid interactions and promote discoidal particle morphology. J Lipid Res. 50(7):1384-94. † - Joint first author
2008
27) Burgess BR†, Dobson RCJ†, Bailey MF, Atkinson SC, Griffin MD, Jameson GB, Parker MW, Gerrard JA & Perugini MA (2008) Structure and evolution of a novel dimeric enzyme from a clinically important bacterial pathogen. J. Biol. Chem. 283:27598-603. † - Joint first author
26) Griffin MDW†, Dobson RCJ†, Pearce FG, Antonio L, Whitten AE, Liew CK, Mackay JP, Trewhella J, Jameson GB, Perugini MA & Gerrard JA (2008) Evolution of quaternary structure in a homotetrameric protein. J. Mol. Biol. 380(4):691-703. † - Joint first author
25) Dobson RCJ, Griffin MD, Devenish SRA, Pearce GF, Hutton CA, Gerrard JA, Jameson GB & Perugini MA (2008) Conserved main-chain peptide distortions: a proposed role for Ile203 in catalysis by dihydrodipicolinate synthase. Protein Sci. 17(12):2080-90. Corresponding author
24) Pearce FG, Dobson RCJ, Weber A, Lane LA, McCammon MG, Squire MA, Perugini MA, Jameson GB, Robinson CV & Gerrard JA (2008) Mutating the tight-dimer interface of dihydrodipicolinate synthase disrupts the enzyme quaternary structure: toward a monomeric enzyme. Biochemistry 47(46):12108-17.
23) Boughton BA, Griffin MDW, O’Donnell PA, Dobson RCJ, Perugini MA, Gerrard JA & Hutton CA (2008) Irreversible inhibition of dihydrodipicolinate synthase by 4-oxo-heptenedioic acid analogues. Bioorg. Med. Chem. 16(23):9975-83.
22) Androulakis S, Schmidberger J, Bate MA, DeGori R, Beitz A, Keong C, Cameron B, McGowan S, Porter CJ, Harrison A, Hunter J, Martin JL, Kobe B, Dobson RCJ, Parker MW, Whisstock JC, Gray J, Treloar A, Groenewegen D, Dickson N & Buckle AM (2008) Federated repositories of X-ray diffraction images. Acta Cryst. D 64:810-4.
21) Devenish SRA, Gerrard JA, Jameson GB & Dobson, RCJ (2008) The high-resolution crystal structure of dihydrodipicolinate synthase from Escherichia coli bound to its first substrate, pyruvate. Acta Cryst. F 64:1092-5. Senior & Corresponding author
20) Boughton BA, Dobson RCJ, Gerrard JA & Hutton CA (2008) Conformationally constrained diketopimelic acid analogues as inhibitors of dihydrodipicolinate synthase. Bioorg. Med. Chem. Lett. 18:460-3.
19) Burgess BR†, Dobson RCJ†, Dogovski C, Jameson GB, Parker MW & Perugini MA (2008) Purification, crystallization and preliminary X-ray diffraction studies to near-atomic resolution of dihydrodipicolinate synthase from methicillin-resistant Staphylococcus aureus. Acta Cryst. F 64:659-61. † - Joint first & Corresponding author
18) Kafela G, Evans G, Griffin MDW, Perugini MA, Gerrard JA, Weiss MS & Dobson RCJ (2008) Crystal structure and kinetic study of dihydrodipicolinate synthase (Rv2753c) from Mycobacterium tuberculosis: an essential gene and protein drug target. Biochem. J. 411(2):351-60. Senior & Corresponding author
17) Dobson RCJ, Atkinson SC, Gorman MA, Newman JM, Parker MW & Perugini MA (2008) The purification, crystallisation, and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Clostridium botulinum. Acta Cryst. F 64:206-8. Corresponding author
16) Mitsakos V, Dobson RCJ, Pearce FG, Devenish SR, Burgess BR, Perugini MA, Gerrard JA & Hutton CA (2008) Inhibiting dihydrodipicolinate synthase across species: towards specificity for pathogens? Bioorg. Med. Chem. Lett. 18:842-4.
2005
15) Dobson RCJ, Devenish SRA, Turner LA, Clifford VR, Pearce FG, Jameson GB & Gerrard JA (2005) The role ofarginine138 in the catalysis and regulation of Escherichia coli dihydrodipicolinate synthase. Biochemistry 44:13007-13. Corresponding author
14) Dobson RCJ, Griffin MDW, Jameson GB & Gerrard JA (2005) The crystal structures of native and (S)-lysine bound dihydrodipicolinate synthase from Escherichia coli with improved resolution shows new features of biological significance. Acta Cryst. D 61:1116-24.
13) Turner JJ, Dobson RCJ, Gerrard JA & Hutton CA (2005) Two new irreversible inhibitors of dihydrodipicolinate synthase: diethyl (E,E)-4-oxohepta-2,5-dienedioate and diethyl-4-oxo-(E)-heptenedioate. Bioorg. Med. Chem. Lett. 15:995-8.
2004
12) Dobson RCJ, Valegard K & Gerrard JA (2004) The crystal structure of three site-directed mutants of Escherichia coli DHDPS: further evidence for a catalytic triad. J. Mol. Biol. 338:329-339.
11) Dobson RCJ, Gerrard JA & Pearce FG (2004) Dihydrodipicolinate synthase is not inhibited by its substrate, (S)-aspartate--semialdehyde. Biochem. J. 377:757-62.
10) Roberts SJ, Morris JC, Dobson RCJ, Baxter CL & Gerrard JA (2004) Two complete syntheses of (S)-aspartate semi-aldehyde and demonstration that D2-tetrahydroisophthalic acid is a non-competitive inhibitor of dihydrodipicolinate synthase. Arkivoc 2004 (Part 10):166-77.
9) Dobson RCJ, Griffin MDW, Roberts SJ & Gerrard JA (2004) Dihydrodipicolinate synthase (DHDPS) from Escherichia coli displays mixed inhibition with respect to its first substrate, pyruvate. Biochimie 86:311-5.
2003
8) Roberts SJ, Morris JC, Dobson RCJ & Gerrard JA (2003) Two efficient syntheses of (S)-aspartate semi-aldehyde appropriate for use in biochemical studies. Bioorg. Med. Chem. Lett. 13:265-67.
Peer reviewed books, book chapters, books edited
7) Lysine Biosynthesis in Bacteria – An Unchartered Pathway for Novel Antibiotic Design. Dogovski C, Atkinson SC, Dommaraju SR, Hor L, Dobson RCJ, Hutton CA, Gerrard JA & Perugini MA (2009) In Volume 11 of the “Biotechnology–Medical Biotechnology: Fundamentals and Modern Development Part I”. pp 116-136
Refereed conference proceedings
6) Perugini MA, Scally SW, Hor L, Voss JE, Taylor NL, Dobson RCJ, Dogovski C, Hutton CA & Gerrard JA (2008) Interface-targeted peptide inhibitors of dihydrodipicolinate synthase: potential use as anti-anthrax agents. Defense Technical Information Center (accepted 21/11/08).
5) Atkinson SC, Dogovski C, Dobson RCJ, Bailey MF, Parker MW & Perugini MA (2008) Structure and stability of dihydrodipicolinate synthase from Clostridium botulinum. Defense Technical Information Center (accepted 21/11/08).
Other forms of dissemination
4) Mills N (2009) Profile of a killer enzyme. Australasian Science March issue (in press)
3) Jones K (2007/8) Protein-protein interactions and enzymology laboratory. What’s New in Lab Technology Vol 18, No. 5, p8.
2) Hannink N (2008) Cocktail to fix bugs that fight drugs. The Voice Vol 3, No. 9, p6
1) Dobson RCJ (2004) Investigating the catalytic and regulatory mechanisms of dihydrodipicolinate synthase. PhD dissertation, University of Canterbury, New Zealand. (Peer reviewed by Prof. Chris Schofield, University of Oxford, UK)