Lab Protocols
Protocol for correcting nonlinearities in continuous flow isotope ratio mass spectrometry (CF-IRMS) measurements
This companion to the February 2012 RCM paper, Correcting for nonlinearity effects of continuous flow isotope ratio mass spectrometry across a wide dynamic range, (Rapid Communications in Mass Spectrometry, 26, 460–468) provides step-by-step instructions for correcting nonlinearities and calibrating delta values over a wide dynamic range in CF-IRMS applications. A worked example with data and R code is included.
Protocol for measuring respiratory electron partitioning using septum-capped vials
This companion to the PC&E paper, A field-compatible method for measuring alternative respiratory pathway activities in vivo using stable O2 isotopes, provides step-by-step instructions for our field-portable method of determining electron partitioning through the alternative oxidase and cytochrome c oxidase respiratory pathways. A worked example with data and R code is included.