External Customers
| Services and Pricing |
|---|
Sequencing Service
We offer two options:
Full service – you provide purified PCR products and PCR primers, we do the rest – $15 per sample.
Capillary separation only – you provided purified sequencing products, we do the rest – $10 per sample.
Genotyping Service
We offer the following option:
Capillary separation only - you supply the internal size standard, you prepare the plate, and we take care of the rest - $5.00 per sample
Please note that we have a 16 sample minimum. That is, if you submit less than 16 samples, these will not be processed until we have obtained additional samples from other internal and/or external clients. The price, however, will remain the same.
| Bookings, request forms, turnaround time |
|---|
Bookings
Please note that we operate on a first come first served policy for all of our customers. However, if your request is urgent we may be able to accommodate you (for an additional charge). Regardless, it is best to contact us to determine our next available booking.
Request forms
Once you have secured a booking, please download the following request form:
Sequencing external request form
Genotyping external request form
Prior to sending your samples, please email the completed request form to us (but also be sure to include a hard copy of the request form with your samples).
Turnaround Time
Our aim is to process your samples as quickly and efficiently as possible without compromising quality. Baring unforeseen circumstances, your results will be returned to you within 24 hours of your booking.
| Sample preparation |
|---|
Sequencing Service
For full sequencing service we require: purified PCR products and PCR primers
PCR products must be free of residual salts, chelating agents (eg, EDTA), proteins, solvents, and buffers. Several PCR purification methods are available. Please choose the product that best suits your needs.
PCR products must be quantified prior to shipment (by eye, Nanodrop, etc). Please provide concentrations in ng/µl.
Please provide PCR primer(s) at a concentration of 3.2 µM.
For capillary separation only service we require: purified sequencing products
Sequencing products must be free of unincorporated fluorescent nucleotides. Several sequencing purification methods are available. Please choose the product that best suits your needs.
Genotyping Service
If you require an internal size standard, we offer GeneScan 500 LIZ for microsatellite genotyping and MapMarker 1000 ROX for AFLP genotyping
Please contact us for advice regarding plate preparation, if need be.
| Getting your results, viewing your results |
|---|
Getting your results
Your results will be sent as attachments (zip files) via email. The maximum file size is approximately 4MB. If you are unable to receive attachments of this size, please contact us and we can arrange for an alternative method of return.
Viewing your results
You may wish to view (and analyse) your results using one of the many freeware programs available on the web.
For sequence data, we suggest Bioedit for PC users and FinchTV for Macintosh users.
For genotype data, we suggest GeneMarker for PC users (to our knowledge there isn’t an equivalent program available for Macintosh users).
| Confidentiality and archive service |
|---|
Confidentiality
We are pleased to assure that your results are confidential are available only to you.
Archive service
Because we maintain an archive, please contact us if you would like to obtain any of your prior results. Please note there may be a small charge for this service.